![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014007:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
AraC-pBAD->sfGFP_8R
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1260
Design Notes
We were looking for the best method to optimize gene expression in E.coli and wanted to check out if there is any influence of the 8R tag for the mRNA stability.
Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with 8R tag to check the translation efficiency.